Embryo Petting and Transgene Infection
Suck it… Spew it… Swallow? Mouth Pipetting and the ins and outs of transgenic mouse production.
It is common practice to mouth pipette transgenic mouse eggs and embryos… I don’t know why, its anathema in every other lab… Perhaps it does free your hands and make for more delicate passaging of fertile rites. But it is also more personal, using oral skills to both kiss and tongue the ova after collection, move the eggs to mix with thawed sperm for fertilization and sort.grade the embryos, afterwards lining them up under the micro-injector and injecting them with trans-genes. Once they are transgenic, its mouth sucking again and the wham bam into the cryo-crib and once thawed again, sucked in and out by mouth again to load the hypodermic and then injected direct into the fallopian ampula of the psuedo.pregnant donor foster mother mouse. I have taught mouth pipetting as a deviant act in the streets, but to see it done in lab, to try it and see if I could taste, smell, accidentally ingest the mouse: ova, sperm, embryos, transgenic embryos? Live life science is more interesting the deeper down the rabbit.mouse antibody intimate holes you get!
“just what exactly is mouth pipetting, dare I ask?”
Like so: insert an open-ended glass capillary tube into your mouth. Place the opposite, tapered end of the tube into a solution of your choice. Microbial stews, blood, cell culture, it is totally your call. With a method that carefully mimics the sucking of a straw, draw a solution upwards through your man-made pipette to your desired volume using the tension created by the reduced air pressure – yes, suction! Maintain the tension with your mouth. Do not suck too hard and inadvertently slurp the solution into your mouth. Careful now. Gently move the pipette end from one vessel and release your precious cargo into yet another vessel.
That is mouth pipetting.”
– Suck It: The Ins and Outs of Mouth Pipetting, Body Horrors, By Rebecca Kreston, Mar 20, 2013 7:15 PM, Discover Magazine Online, https://www.discovermagazine.com/the-sciences/suck-it-the-ins-and-outs-of-mouth-pipetting
Embryo Petting
The following video is of a mouse embryo transgenic initialization. The embryo is shown affection before transgenic CrispR infectious microinjection. This germline alteration and petting directly after fertilization and moments before cryopreservation in liquid nitrogen. The video incorporates a traditional Jewish Sephardic lullaby. The CrispR Guide Construct was microinjected into the E 3.9 stage of the blastocyst, https://embryology.med.unsw.edu.au/embryology/index.php/Mouse_Stages
Video, Microinjection and Petting: Adam Zaretsky
Duerme, duerme vocals: Dalila Honorato
Lyrics:
Durme, durme ijiko de madre
Durme, durme sin ansia i dolor
Durme, durme sin ansia i dolor
Sienti djoia palavrikas de tu madre
Las palavras de Shema Israel
Las palavras de Shema Israel
Durme, durme ijiko de madre
Kon ermozura de Shema Israel
Kon ermozura de Shema Israel
Translation:
Sleep, sleep; mother’s little boy
Free from worry and from pain,
Free from worry and from pain.
Listen to the joy of your mother’s words,
The words of Shema Israel(*),
The words of Shema Israel.
Sleep, sleep; mother’s little boy
With the beauty of Shema Israel,
With the beauty of Shema Israel.
(*) Jewish prayer.
Afagando o embrião
Vídeo de embrião de rato e manipulação com agulha antes da criopreservação incorporando uma canção de embalar da tradição judaica sefardita.
Tradução:
Dorme, dorme; menino da mãe
Livre de ansiedades e dor,
Livre de ansiedades e dor.
Escuta o carinho nas palavras da mãe,
As palavras de Shemá Israel(*),
As palavras de Shemá Israel.
Dorme, dorme; menino da mãe
Na beleza de Shemá Israel,
Na beleza de Shemá Israel.
(*) Oração judaica.
